Differences

This shows you the differences between two versions of the page.

--- geneonyxmar302012:start [2015/05/06 03:24] – magiero
+++ geneonyxmar302012:start [2015/05/06 03:53] (current) – magiero
@@ Line 38: / Line 38: @@
 ==== - Summary ====
+In my words\\
+• You have four small chambers (per "feature"?), each containing, A,C,G,T, respectively.\\
+• In each of these chambers you pump your target DNA and a primer (and some enzyme).\\
+• With this we are looking for a SNP in the n+1 position of the target.\\
+• Ostensibly the primer (of length n) binds to the DNA at the predetermined spot (defined by the sequence of the primer itself).\\
+• The chamber containing the nt that complements the SNP (in the n+1 position) will then undergo a hydrolisis reaction that drops the pH and is detected by an ISFET.\\
+• The ISFET's (one per chamber) are **cleverly interfaced** (__this is basically the invention__) to four differential amps (based on knowledge of allele possibilities in mammals) which produce signal combinations such that the type of SNP encountered can be identified.\\
+• This arrangement reduces the effect of offsets and the need for extensive, high-resolution sampling.
 ==== - Detailed Description ====