Differences

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--- oxforddec192007:start [2016/02/23 01:31] – magiero
+++ oxforddec192007:start [2016/02/23 03:21] (current) – magiero
@@ Line 49: / Line 49: @@
 ==== - Forming a Layer of Amphiphilic Molecules [0152] - [0159] ====
+• [0158] Method applicable to any amphiphilic molecules, not just lipids.\\
+• [0159] Aqueous solution 10 can be of broad range.  Should be "physiologically acceptable", buffered to pH of 3 to 11, depending on amphiphilic molecules and final application of layer 11. An example of aqueous solution 10 is 10mM PBS containing 1M NaCl with pH of 6.9.\\
 ==== - Method of Using Apparatus 1 [0160] - [0183] ====
 {{ layerform1.png?300}}
-• [0161] First, a pre-treatment coating 30 is applied to the body 2 across the recess 5.  The pre-treatment coating 30 is a hydrophobic fluid which modifies the surface of the body 2 surrounding the recess 5 to increase its affinity to the amphiphilic molecules.\\
+• [0161] First, a pre-treatment coating 30 is applied to the body 2 across the recess 5.  The pre-treatment coating 30 is a **hydrophobic fluid** which modifies the surface of the body 2 surrounding the recess 5 to increase its affinity to the amphiphilic molecules.\\
+• [0162] 30 is usually an **organic substance** with long chain molecules in an **organic solvent**.\\
+**Organic Substance**
+  * n-decane
+  * hexadecane, etc.
+**Organic Solvent**
+  * pentane
+  * hexane
+  * heptane, etc.
+• [0163] Example mixtures include:
+  * 0.3% hexadecane in pentane
+  * 1.0% hexadecane in pentane
+  * 10% hexadecane in pentane
+  * 1% hexadecane + 0.6 mg/ml DPhPC lipid in pentane
+• [0164] Pre-treatment may be applied in "any suitable manner", for example by capillary pipette: __**SO IT'S NOT AUTOMATED?!?!?!**__\\
 • [0168] After application of the pre-treatment coating 30, the aqueous solution 10 is flowed across the body 2 to cover the recess 5.  This step is performed with the amphiphilic molecules added to the aqueous solution 10.
 • [0169] The layers 11 have high resistance providing highly resistive electrical seals, having an electrical resistance of 1 GΩ or more, typically at least 100 GΩ which, for example, enables high-fidelity stochastic recordings from single protein pores.\\
@@ Line 66: / Line 83: @@
 {{ layerform2.png?200}}
 • Want to avoid coating on electrode.\\
+• [0201] **Electro-Wetting**, apply voltage to electrodes 21 and 24 to blow-off the pre-treatment covering electrode 21.\\
 ==== - Example 1 [0208] - [0218] ====