Differences

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--- oxfordfeb202007:start [2015/02/01 16:55] – magiero
+++ oxfordfeb202007:start [2015/11/06 02:48] (current) – magiero
@@ Line 60: / Line 60: @@
 {{ readerfig26.png?400}}\\
 • [0250] The reference voltage of 62 is set by a LPF'd PWM waveform.  This is a convenient way of getting digital signals to set an analog level.\\
-• [0252] If you want to array it is suggested that you just use an **analog multiplexer**.\\
+• [0252] If you want to array it is suggested that you just use an **analog multiplexer**.  That is **multiple analog** circuits 93 and 94 would be used and multiplexed into a single microcontroller.\\
 ==== - How a Reader can Analyze [0257]–[0267] ====
@@ Line 66: / Line 66: @@
 • [0264] The state of the cell may be detected on the basis of **predetermined thresholds** or **adaptive thresholds**.\\
 • [0266] Also store the **time duration** of a state.\\
-• [0266] This could be indicative of the number of binding events.  For example what if you are sensing multiple pores (with one channel) the multiple binding events may combine to appear at signals with variable time durations.\\
+• [0266] This could be indicative of the number of binding events.  For example what if you are sensing multiple pores (with one channel) the multiple binding events may combine to appear as signals with variable time durations.\\
+• This processing strategy is referenced in [[oxfordapr202009b:start | Dec. 01, 2009 patent]].
 ==== - State Monitoring Algorithm Example [0268]–[0280] ====
-• [0270] With the reader empty (and essentially capacitive) apply a 20-mV amplitude 50-Hz triangular waveform (centred at 100-mV) to make sure that you measure the appropriate (20-pA) square wave current.\\
+{{ stateflow.png?300}}
-• [0271] After inserting a dry cell a similar (albeit with changed amplitude) current should be measured.\\
+• [0270] With the reader empty (and essentially capacitive since there is no cell placed between the reader's electrodes) apply a 20-mV amplitude 50-Hz triangular waveform (centred at 100-mV) to make sure that you measure the appropriate (20-pA) square wave current.  This is **state S1**.\\
+{{ appliedcontrolvoltage.png?300 }}\\
+{{ drycurrents.png?300 }}
+• [0271] After inserting a dry cell a similar (albeit with changed amplitude) current should be measured.  Expect say a 25% increase in the measured current.\\
 • [0273] With the ionic fluid introduced into the cell a large current should flow thus saturating the amplifier current output and therefore indicating that the cell now contains ionic fluid.\\
+{{ nobilayercurrent.png?300 }}
 • [0274] When the lipid bilayer forms you should again see a drop in the current (you have re-established a capacitance).  Typically 250-pA amplitude centred at 0 pA.  Typical DC resistance is 10 GΩ.\\
+{{ bilayercurrent.png?300 }}
 • [0277] Protein insertion into the bilayer can be sensed.  **So you know how many pores you have in your bilayer as they interface with it**.
+{{ poreinsertioncurrent.png?300 }}
+{{ analytebindingcurrent.png?300 }}