Differences

This shows you the differences between two versions of the page.

--- oxfordfeb202007:start [2015/02/01 17:18] – magiero
+++ oxfordfeb202007:start [2015/11/06 02:48] (current) – magiero
@@ Line 67: / Line 67: @@
 • [0266] Also store the **time duration** of a state.\\
 • [0266] This could be indicative of the number of binding events.  For example what if you are sensing multiple pores (with one channel) the multiple binding events may combine to appear as signals with variable time durations.\\
+• This processing strategy is referenced in [[oxfordapr202009b:start | Dec. 01, 2009 patent]].
 ==== - State Monitoring Algorithm Example [0268]–[0280] ====
@@ Line 72: / Line 73: @@
 {{ stateflow.png?300}}
-• [0270] With the reader empty (and essentially capacitive since there is no cell placed between the reader's electrodes) apply a 20-mV amplitude 50-Hz triangular waveform (centred at 100-mV) to make sure that you measure the appropriate (20-pA) square wave current.\\
+• [0270] With the reader empty (and essentially capacitive since there is no cell placed between the reader's electrodes) apply a 20-mV amplitude 50-Hz triangular waveform (centred at 100-mV) to make sure that you measure the appropriate (20-pA) square wave current.  This is **state S1**.\\
-• [0271] After inserting a dry cell a similar (albeit with changed amplitude) current should be measured.\\
+{{ appliedcontrolvoltage.png?300 }}\\
+{{ drycurrents.png?300 }}
+• [0271] After inserting a dry cell a similar (albeit with changed amplitude) current should be measured.  Expect say a 25% increase in the measured current.\\
 • [0273] With the ionic fluid introduced into the cell a large current should flow thus saturating the amplifier current output and therefore indicating that the cell now contains ionic fluid.\\
+{{ nobilayercurrent.png?300 }}
 • [0274] When the lipid bilayer forms you should again see a drop in the current (you have re-established a capacitance).  Typically 250-pA amplitude centred at 0 pA.  Typical DC resistance is 10 GΩ.\\
+{{ bilayercurrent.png?300 }}
 • [0277] Protein insertion into the bilayer can be sensed.  **So you know how many pores you have in your bilayer as they interface with it**.
+{{ poreinsertioncurrent.png?300 }}
+{{ analytebindingcurrent.png?300 }}