Differences

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--- oxfordapr202009a:start [2015/02/04 16:58] – magiero
+++ oxfordapr202009a:start [2015/02/05 04:24] (current) – magiero
@@ Line 44: / Line 44: @@
 • [0072] However, it is noted that the quality of performance of wells 21 becoming unacceptable is relatively rare.\\
-==== - Redundancy and Efficiency [0073] - [] ====
+==== - Redundancy and Efficiency [0073] - [0090] ====
 • [0074] The insertion of membrane proteins into a lipid bilayer 26 is a random process that follows Poisson statistics. This means that even when the average number of membrane proteins per well 21 is one, a significant number of wells 21 may have none, two or more membrane proteins inserted, and these wells 21 are then not useful. For example, it is found that in a particular embodiment the maximum probability for finding just one membrane protein in a well 21 is about 36%, and this is only achieved if conditions are optimal.  A greater or lesser membrane protein concentration quickly results in a reduction of useable wells 21 (especially a lesser exposure). **Current estimates for efficiency which is likely to be achieved in practical embodiments are about 20%**.\\
 • [0076] **Lipid bilayers 26 are formed with an efficiency which may be assumed to approach 100%** for current purposes. Membrane proteins are then inserted using a solution whose concentration and exposure time is adjusted to give a mean number of membrane proteins per well 21 near to one. Because the wells 21 might not in practice have a lipid bilayer 26 of the same size, their capture efficiencies will vary. This combines with Poisson statistics to give a spread in the number of membrane proteins per well 21. The apparatus 1 characterises the sensor device 2 to detect which wells 21 have active, useful membrane proteins.\\
+• [0078] The mean of the pore Poisson distribution depends on
+  - area of the lipid bilayer
+  - concentration of membrane proteins in the solution
+  - time for which lipid bilayer is exposed to the membrane protein solution
+• [0087] **Cross-bar switch** is possible, but much more complex.
+==== - Modified Detection Circuit [0091] - [0098] ====
+{{ detector3.png?400}}
+• [0092] In this one you can unblock the pores (supply an inverted potential) without affecting other wells 21.\\
+• [0095] Now provide a second switch arrangement 48 that can connect up the unblocking bias source 47 to the pore in question.\\
+• These switches are referenced in [[oxfordapr202009b:start | Dec. 01, 2009 patent]].