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brg336_-_medical_devices_teaching_lab:laboratory_materials

Laboratory Materials

Cell Culture Medium

  1. DMEM (from Gibco) / “2* 500ml=1L”
  2. Glutamax / 100ml
  3. penicillin/streptomycin / 100ml
  4. fetal bovine serum (FBS) / 100ml

Cell culture and Passage material

  1. Sterile pipet 5ml / 200 ea
  2. Sterile pipet 10ml / 200ea
  3. Finntip™ Pipette Tips 1000 μL-Sterile / “Pack of 1000”
  4. Finntip™ Pipette Tips 10 μL-Sterile / “Pack of 1000”
  5. Finntip™ Pipette Tips 200 μL-Sterile / “Pack of 1000”
  6. Pipet gun / 1ea
  7. Cell culture Flask 25T 30 ea
  8. “Cell culture Flask 75T (Corning® CellBIND® Surface cell culture flasks)” / 5ea
  9. DPBS Solution (without calcium and magnesium) / 500ml
  10. Sigma-Aldrich® anti-static disposable unisex lab coat
  11. Trypsin 1% (removing cell during passage) / 100 ml

Phalloidin - Dapi Staining

  1. 4%paraformaldehyde 100g
  2. Triton X-100 50ml
  3. diamidino-2- phenylindole (DAPI) 1 mL
  4. Phalloidin-TRITC 0.01mg
  5. PBS 1L

MTS Assay

  1. 96 well plate 25ea
  2. 12 well plate 10ea
  3. 24 well plate 5ea
  4. MTS Assay Kit (Cell Proliferation) (Colorimetric) 250Test

Contact lens (cell culture substrate)

  1. Acuvue Oasys 12 Pack “12 Pack”

Cell Line

  1. Human Dental Pulp Stem Cells (hDPSC) Cells “1000000 cells”
  2. DPSC - Human Dental Pulp Stem Cells Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells

Electrode

  1. Screen Printed Gold Electrodes
brg336_-_medical_devices_teaching_lab/laboratory_materials.txt · Last modified: 2024/12/17 15:51 by gcayala

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