Cell Culture Medium

1. DMEM (from Gibco) / “2* 500ml=1L 164$”
2. Glutamax / 100ml=108$
3. penicillin/streptomycin / 100ml=42$
4. fetal bovine serum (FBS) / 100ml=340$

Cell culture and Passage material

1. Sterile pipet 5ml / 200 ea=109$ - Sterile pipet 10ml / 200ea=130$
2. Finntip™ Pipette Tips 1000 μL-Sterile / “Pack of 1000=221$”
3. Finntip™ Pipette Tips 10 μL-Sterile / “Pack of 1000=224$”
4. Finntip™ Pipette Tips 200 μL-Sterile / “Pack of 1000=375$”
5. Pipet gun / 1ea=706$
6. Cell culture Flask 25T 30 ea
7. “Cell culture Flask 75T (Corning® CellBIND® Surface cell culture flasks)” / 5ea=63$ - DPBS Solution (without calcium and magnesium) / 500ml=33$
8. Sigma-Aldrich® anti-static disposable unisex lab coat
9. Trypsin 1% (removing cell during passage) / 100 ml=51$

Phalloidin - Dapi Staining

1. 4%paraformaldehyde 100g=85$ - Triton X-100 50ml=46$
2. diamidino-2- phenylindole (DAPI) 1 mL=104$ - Phalloidin-TRITC 0.01mg=320$
3. PBS 1L=48.8$

MTS Assay

1. 96 well plate 25ea=100$ - 12 well plate 10ea=50$
2. 24 well plate 5ea=60$ - MTS Assay Kit (Cell Proliferation) (Colorimetric) 250Test=180$

Contact lens (cell culture substrate)

1. Acuvue Oasys 12 Pack “12 Pack=58.09$”