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brg336_-_medical_devices_teaching_lab:laboratory_materials

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Laboratory Materials

Cell Culture Medium

  1. DMEM (from Gibco) / “2* 500ml=1L”
  2. Glutamax / 100ml
  3. penicillin/streptomycin / 100ml
  4. fetal bovine serum (FBS) / 100ml

Cell culture and Passage material

  1. Sterile pipet 5ml / 200 ea
  2. Sterile pipet 10ml / 200ea
  3. Finntip™ Pipette Tips 1000 μL-Sterile / “Pack of 1000”
  4. Finntip™ Pipette Tips 10 μL-Sterile / “Pack of 1000”
  5. Finntip™ Pipette Tips 200 μL-Sterile / “Pack of 1000”
  6. Pipet gun / 1ea
  7. Cell culture Flask 25T 30 ea
  8. “Cell culture Flask 75T (Corning® CellBIND® Surface cell culture flasks)” / 5ea=63$
  9. DPBS Solution (without calcium and magnesium) / 500ml
  10. Sigma-Aldrich® anti-static disposable unisex lab coat
  11. Trypsin 1% (removing cell during passage) / 100 ml

Phalloidin - Dapi Staining

  1. 4%paraformaldehyde 100g
  2. Triton X-100 50ml
  3. diamidino-2- phenylindole (DAPI) 1 mL
  4. Phalloidin-TRITC 0.01mg
  5. PBS 1L

MTS Assay

  1. 96 well plate 25ea
  2. 12 well plate 10ea
  3. 24 well plate 5ea
  4. MTS Assay Kit (Cell Proliferation) (Colorimetric) 250Test=180$

Contact lens (cell culture substrate)

  1. Acuvue Oasys 12 Pack “12 Pack”

Cell Line

  1. Human Dental Pulp Stem Cells (hDPSC) Cells “1000000 cells”
  2. DPSC - Human Dental Pulp Stem Cells Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells

Electrode

  1. Screen Printed Gold Electrodes
brg336_-_medical_devices_teaching_lab/laboratory_materials.1734450666.txt.gz · Last modified: 2024/12/17 15:51 by gcayala

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